Jose Cuenca

4 Questions 18 Answers 0 Followers

Questions related from Jose Cuenca

Is it possible to determine which one of the two genomes is the ancestor of the other?

I have the complete sequence of two genotypes and I would like to infer which one is the origin to the other. Probably the analysis should be based on SNPs and Indels? Is there any tool to predict...

10 October 2016 3,584 2 View

Which strategy would be the best to test the role of putative regulatory motifs in the upstream sequence of a plant gene?

Hi. I would like to check the differential expression of two versions of this gene, one of this containing an insertion in the upstream. I planned to transform Arabidopsis with the whole gene (not...

10 October 2016 6,044 8 View

Could variants (SNP) within an Indel explain the variation in gene expression across varieties?

Hi. This is to anyone who has ever observed the same. We found a large Indel (400bp) within a known gene. When the insertion is not present (deletion), the gene is expressed (like a functional...

09 September 2016 1,064 12 View

Preparing RNA-seq libraries, good concentration using Qubit assay but nothing appears as I run the gel, except the ladder?

Hi. I'm preparing RNA-seq libraries and I measure the concentration using Qubit assay. I got good values (around 6-8), but when I run the gel, nothing appears, only the ladder. Does it mean I...

03 March 2016 6,754 7 View