Jose Sevilla

2 Questions 8 Answers 0 Followers

Questions related from Jose Sevilla

TaqMan qPCR Assay Using a StepOnePlus - Has anyone experienced Ct values increasing at random for established controls?

Greetings. I'm running a TaqMan assay on the StepOnePlus R-T PCR System, using the TaqMan Fast Advanced Master Mix. I validated an assay that gives me very precise Ct values for positive controls...

18 October 2017 6,547 2 View

How Do I Detect a Low Copy Target Using TaqMan PCR?

Good morning. I've been trying to detect a low copy bacterial target using TaqMan Universal PCR Master Mix and specific primers/probe. Unfortunately, I only have about a 50% detection rate for...

05 June 2017 7,482 5 View