José Luis Sánchez-López

2 Questions 2 Answers 0 Followers

Questions related from José Luis Sánchez-López

How to make protocol adjustments for multiplex PCR using Phusion Polymerase?

I want to perform a multiplex PCR using two sets of primers, with one amplicon measuring 71 bp and the other measuring 104 bp. I have already designed the primers so that their Tm values are very...

4 weeks ago 5,586 4 View

When doing site directed mutagenesis, my insert disappears and instead I get a new insert, how is that possible?

Hello, So in my lab we've been doing site-directed mutagenesis for a long time using the same kit (Agilent's Quick Flash) and it's always worked wonders, but a few months ago I did one and when...

03 June 2025 1,831 2 View