José Lozano

2 Questions 9 Answers 0 Followers

Questions related from José Lozano

How to avoid smiling in Phos-Tag acrylamide gels?

We are trying to optimize SDS-PAGE conditions for Phos-Tag-containing gels but so far we´ve been unable to avoid a consistent smiling effect. Any hints/tips on how to solve this issue? Thanks

23 May 2012 1,519 17 View

No upshifted bands in Bis-Tris precast gels

Hi everyone. After switching from using traditional Tris-Glycine SDS-PAGE to Bis-Tris precast gels we no longer observe by Western blot the typical upshifted bands corresponding to phosphorylated...

01 January 1970 8,635 1 View