Can I use alpha MEM with FBS for osteoclast differentiation? And will trypsin usage decrease the ability of Raw cells differentiation?

I am using murine sRANKL for osteoclast differentiation in Raw 264.7. I would like to know if the differential media will have FBS or not. And I have been using Trypsin digestion for subculturing...

18 October 2022 4,402 2 View