Jorrit De Waele

3 Questions 10 Answers 0 Followers

Questions related from Jorrit De Waele

Protocol to dissociate spheroids into single cells for flow cytometry?

Dear all, I am working with spheroids of U251 and U87 glioblastoma cells. Now, I would like to dissociate the spheroids for single cell flow cytometry analysis. However, either the spheroids are...

19 March 2018 2,032 7 View

Which markers are best to differentiate between human monocytes and macrophages?

In order to differentiate between human monocytes and (monocyte-derived) macrophages, which markers can best be used in flow cytometry?

30 September 2014 1,417 30 View

Does anybody know a positive magnetic NK cell isolation kit?

I would like to isolate NK from PBMC by positive magnetic selection to obtain an untouched, NK-less PBMC fraction. The only commercial kits with positive NK selection I came across, used a...

10 June 2014 3,067 5 View