Jorrit De Waele

4 Questions 11 Answers 0 Followers

Questions related from Jorrit De Waele

Protocol to dissociate spheroids into single cells for flow cytometry?

Dear all, I am working with spheroids of U251 and U87 glioblastoma cells. Now, I would like to dissociate the spheroids for single cell flow cytometry analysis. However, either the spheroids are...

19 March 2018 2,033 7 View

Which markers are best to differentiate between human monocytes and macrophages?

In order to differentiate between human monocytes and (monocyte-derived) macrophages, which markers can best be used in flow cytometry?

30 September 2014 1,419 30 View

How can I get rid of contaminating T cells during monocyte enrichment via adherence?

I want to isolate monocytes from peripheral blood mononuclear cells via adherence. For this, firstly the peripheral blood mononuclear cells are isolated from buffy coats using Ficoll density...

15 August 2014 1,296 6 View

Does anybody know a positive magnetic NK cell isolation kit?

I would like to isolate NK from PBMC by positive magnetic selection to obtain an untouched, NK-less PBMC fraction. The only commercial kits with positive NK selection I came across, used a...

10 June 2014 3,069 5 View