Jorge Versicolor

3 Questions 2 Answers 0 Followers

Questions related from Jorge Versicolor

How to reduce droplet merging in droplet digital PCR (dPCR) ?

Hi everyone. I am performing PCR in droplets of 20um of diamater and my protocol is a classical PCR protocol, oil HFE 7500 and surfactant is PicoSurf 3%. I leave like 20uL of oil on the bottom of...

10 October 2019 9,075 3 View

How to avoid self ligation of large inserts during clonning?

Hello everyone, I've been trying to perform a ligation for the last couple of weeks and I just get empty plasmids. The insert is pool of fragments amplified by PCR with an average length of 3kb...

04 April 2019 9,825 6 View

Is it possible to amplify by PCR a unique fragment using MDA product as template?

I am trying to amplify by PCR a fragment (2kb) from a DNA template which is the product of MDA reaction. This MDA reaction is previousliy performed with plasmids as template and all of them...

01 January 2019 3,985 1 View