Jorge Enrique Vazquez Buchelli

4 Questions 1 Answers 0 Followers

Questions related from Jorge Enrique Vazquez Buchelli

Why do I have a Jagged line on my qPCR amplification?

I am performing a qPCR standardization of some MGB probes. I have tried just a few times but I don't want to waste reagents, every time I tried the assay, there is a jagging line. I am thinking...

11 November 2019 7,195 3 View

Why im having low probe specificity on Fluorescent in situ hybridization in bacterial suspension?

I am using specific probes for detecting and quantify bacteria with FACS, however in spite, it is supposed to work (Previous studies and In silico analysis of the probes), I only get high...

10 October 2019 9,941 2 View

Can a probe work with less Tm than the primers?

I found 2 interesting articles for the detection of bacteria at Phylum level with MGB probes, claiming that they work. I want to use that primers however when I use the oligoanalyzer tool, it...

07 July 2019 3,002 4 View

Do FISH (Fluorescent in situ hybridization) probes need a quencher?

Can I only label mltiple intern sites in the probe?

12 December 2018 7,827 3 View