Jorge Ferreira

3 Questions 6 Answers 0 Followers

Questions related from Jorge Ferreira

Why monoclonal and polyclonal antibodies, targetting the same protein, reveal immunoblotting bands with different molecular weights?

Hello, I am using two different antibodies specific for the same membrane protein: one of them is monoclonal and the other polyclonal. However, when I reveal the PDVF membrane the results are...

06 June 2018 1,336 4 View

Main reasons for low overall protein yield with Mem-PER Plus Kit?

Hello, I intend to apply Mem-PER Plus Extraction Kit (ThermoFisher) for membrane protein extraction from LNCaP cell culture (adherent mammalian cells). I tried to use this kit exactly as...

03 March 2018 5,240 2 View

Which is the best strategy for specific protein quantification?

After performing extraction followed by chromatographic purification, I intend to discover the concentration of my target protein.

02 February 2018 4,456 10 View