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Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...
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Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...
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Hello everyone, I've been trying to perform a ligation for the last couple of weeks and I just get empty plasmids. The insert is pool of fragments amplified by PCR with an average length of 3kb...
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