Jordi Argilaguet

4 Questions 10 Answers 0 Followers

Questions related from Jordi Argilaguet

Can anyone help me with hibridoma cell growing?

The medium I use is: IMDM, 10%FCS, P/S, 7.5% Sodium Bicarbonate, 0.1mM b-mercaptoethanol Cells double their number in 24h after thawing, but then hey do not expand more (actually the number...

05 May 2015 9,675 8 View

FC receptors blockade for cytometry?

I used to perform intracellular staining for T cells from mouse spleen without blocking FC Receptors and I had no problems (isotype controls). Is it better to block them when analyzing B cells...

06 June 2013 8,126 19 View

Is it important to add HEPES to primary cell cultures for proliferation assays?

Is there any difference in HEPES requirement when working with mice or human cells?

12 December 2012 6,285 12 View

Spleenocytes proliferation and cell death.

When doinf CFSE-proliferation assay with spleen cells from mice I get more then 60% of cell death after 3 days, specially in non-stimulated wells. Same protocol works perfect with PBMCs from...

05 May 2012 2,142 10 View