Jordi Argilaguet

2 Questions 6 Answers 0 Followers

Questions related from Jordi Argilaguet

Is it important to add HEPES to primary cell cultures for proliferation assays?

Is there any difference in HEPES requirement when working with mice or human cells?

11 December 2012 3,750 12 View

Spleenocytes proliferation and cell death.

When doinf CFSE-proliferation assay with spleen cells from mice I get more then 60% of cell death after 3 days, specially in non-stimulated wells. Same protocol works perfect with PBMCs from...

07 May 2012 3,667 9 View