Jordan Ross

3 Questions 11 Answers 0 Followers

Questions related from Jordan Ross

How can I explain a non-specific band on a western blot that appears with several different probings of different species?

I have been reprobing the same two blots most of the week and keep noticing a band in the 50-75kD range that doesn't correspond to anything I'm probing for. What's even more odd is that it appears...

09 September 2014 5,354 15 View

Can I use sodium azide as an alternative to stripping?

I want to reprobe my blot but don't want to strip unless I have to (so as to avoid losing protein - when I strip I use the pH2.5 glycine method which is supposed to be the preferred method for...

08 August 2014 7,078 7 View

When should you use TBS vs TBST to dilute antibodies for Western Blot?

I'm fairly new to Western Blotting but was first taught to dilute my antibodies in my blocking buffer (which is 5% NDM in TBST). Recently, during some troubleshooting, I was advised to get out of...

08 August 2014 9,111 11 View