Jordan B Jahrling

5 Questions 13 Answers 0 Followers

Questions related from Jordan B Jahrling

Can anyone advise me on a concentration and treatment time to use an MMP9 inhibitor in cell culture?

I have obtained a commercially available MMP9 inhibitor that I intend to use in various cell culture experiments. Unfortunately, no information is provided regarding an appropriate concentration...

12 December 2015 5,189 7 View

Could anyone recommend brain regions to look at to investigate tight junctions?

I am planning to do an IHC study from mouse coronal brain sections and will be looking at various tight junction proteins. I of course realize that endothelium is present throughout the brain but...

02 February 2015 461 6 View

Is there any blood flow to the occluded region during MCAO?

When performed experimentally for stroke/ischemia/reperfusion models, does MCAO completely prevent blood flow to the occluded side or just significantly reduce it by cutting off the main source?...

01 January 2015 8,891 5 View

Does anyone have a protocol for growing bEnd3 cells on Corning Transwell inserts?

I am attempting to do some in vitro blood-brain barrier experiments using Corning Transwell pore inserts. Unfortunately, I have not had any luck getting my bEnd3 (mouse brain endothelial) cells to...

12 December 2014 5,243 14 View

Is there a known drug or chemical that can be given to mice to induce blood-brain barrier leakage?

I am looking at the effects of a compound on blood-brain barrier integrity. Preliminary results indicate that the compound improves tight junction expression in aged mice. I am looking for a way...

11 November 2014 1,980 9 View