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I'm wondering what kind of integrity the cells have afterwards. I'm using cell tak to adhere bovine lymphocytes and then interrogating their OCR and ECAR with the seahorse instrument.
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I collected blood from a dairy cow and used a typical PBMC isolation protocol. Briefly, I diluted the sample 1:1 with PBS and centrifuged, removed the buffy coat and added it to 30ml PBS and then...
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