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I want to reprobe my blot but don't want to strip unless I have to (so as to avoid losing protein - when I strip I use the pH2.5 glycine method which is supposed to be the preferred method for...
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I'm fairly new to Western Blotting but was first taught to dilute my antibodies in my blocking buffer (which is 5% NDM in TBST). Recently, during some troubleshooting, I was advised to get out of...
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