Jordan Boeck

3 Questions 1 Answers 0 Followers

Questions related from Jordan Boeck

Does Enzyme Concentration Affect Km?

Hello, I am using Trypsin to determine its Km and Vmax values against casein from bovine milk. I know higher enzyme concentrations increase the Vmax value, but does more trypsin also affect the...

05 April 2024 8,489 3 View

Michaelis-Menten Equation and Substrate Concentration?

Hello, Background: I am trying to find the Vmax and Km values for 0.25% trypsin and the substrate casein. During optimization, I have tried different time points (1-15 minutes) and different...

02 April 2024 1,531 1 View

What do I do if my Bradford Reagent yields underestimated protein concentration for measuring trypsin?

I use 0.25% trypsin (2.5g/L) as a positive control in my protease assays. I'm trying to measure the stability of different proteases overtime and at different temps. When I measure the...

31 March 2024 8,452 0 View