Joppe Wesseling

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Questions related from Joppe Wesseling

Does either freeze/thawing or DMEM (with pyruvate, gentamycin and glutamax) have an effect on the IL-1B concentration?

For cell stimulation with Silica I am measuring IL-1B with ELISA. To control for possible absorption of IL-1B by the silica I measure the effect of different concentrations of silica on the IL-1B...

12 April 2023 1,754 0 View

Do you have a explanation for less phagocytosis at longer incubation times?

I performed a phagocytosis assay of PBMC's with K. pneumoniae with moi's of 2:1 and 1:1 for 90, 30 and 15 minutes. The assay was done using 5-DTAF labelled K. pneumoiae and analysed with flow...

23 March 2023 7,624 3 View