Jooeun Lee

2 Questions 1 Answers 0 Followers

Questions related from Jooeun Lee

Can IGEPAL CA-630(Nonidet P-40) permeablize nuclear membrane, chloroplast membrane and mitochondrial membrane?

I used 3 % IGEPAL CA-630 to permeablize Arabidopsis thaliana leaf cell membrane during Immunofluorescence. And It doesn't seem like IGEPAL CA-630 is able to permeablize organelle membranes(such as...

02 November 2019 6,166 0 View

Why is 70% ethanol is used instead of 100% ethanol during dehydration process for immunocytochemistry?

I'm conducting immunogold-staining with LR white. Here is the protocol of dehydration Conventional: 50% EtOH/50% water ? 15 min 70% EtOH/30% water ? 15 min 90% EtOH/10% water ? 10 min 100%...

17 October 2019 2,685 3 View