Jong Won Yoon

6 Questions 7 Answers 0 Followers

Questions related from Jong Won Yoon

How can I estimate the purity of a recombinant protein after purification process using size-exclusion chromatography (no standard product exists)?

I produced a recombinant chimeric protein from CHO cells and purified it using size-exclusion chromatography(SEC). Thankfully, I could get a clear peak for my target protein and separate it from...

24 April 2019 4,504 12 View

(PRNT assay) The number of plaques decreases as the serum concentration increases in negative control group. Why? Matrix effect??

I'm working on vaccine study I injected a vaccine candidate and PBS(mock) into mice (2 times with 2-week interval), and collect blood from them to get serum samples. To test the vaccine...

27 February 2018 4,838 5 View

What is the difference between sucrose gradient ultracentrifugation and sucrose cushion ultracentrifugation in virus purification process?

I am trying to purify zika virus from cultured vero cells. Since 2% of FBS was added to the culture medium, I need to remove FBS from the virus supernatant. For this work, I am using sucrose...

01 November 2017 8,033 2 View

Can anyone tell me what's wrong with my plaque assay?

Hi, I am working on measuring the activity of a certain virus. From last week, when I do plaque assay, a big hole is formed in the center of the well. I've never seen this before, but the same...

26 October 2017 118 9 View

I lost virus during the purification process(specifically after sucrose gradient ultracentrifugation). What is the reason? and what can I try?

I am purifying the virus with the sucrose gradient method, but the result is not good. 1. The virus supernatant was first concentrated by PEG precipitation method. 2. I confirmed the successful...

16 October 2017 7,619 2 View

I am working on zika virus concentration and inactivation. What happens if I inactivate the virus and then go on sucrose gradient purification step?

Hi, I am working on virus concentration and purification. I have read papers about sucrose gradient purification and the use of formalin to inactivate a virus. And here is my question. What would...

22 September 2017 6,580 1 View