Jonathan Greenberg

2 Questions 3 Answers 0 Followers

Questions related from Jonathan Greenberg

Should I use PIPES or MOPS as a buffer in agar under anaerobic conditions with 5% CO2?

I am about to begin cultivation of mixed bacterial communities under anaerobic conditions with 5% CO2, should I use PIPES or MOPS as a buffer? The concern is regarding which is more appropriate...

01 January 2018 4,199 0 View

Would nested PCR on a 16S HV region reduce background/contaminant environmental 16S genes?

My lab was having a discussion on which type of PCR to do to best amplify our 16S genes from our genomic DNA extractions. After my mentor explained what nested PCR was I recommended it based on...

02 February 2017 8,026 1 View