Jonas De Saeger

3 Questions 3 Answers 0 Followers

Questions related from Jonas De Saeger

Does anyone have experience using cryopreservation of plant suspension cells?

I'm having some problems with cryopreservation of BY-2 suspension cells. I followed the protocol from "Initiation, growth and cryopreservation of plant cell suspension cultures, Mustafa et al....

01 January 2019 6,380 1 View

Which (MoClo) acceptor vector is recommended for the transfection to protoplasts?

Dear all, I would like to transfect protoplasts with constructs made using the MoClo technique, but it seems that the vectors in my toolkit are specifically made for Agrobacterium-mediated...

12 December 2016 4,654 6 View

Does anyone have experience with cloning methods in synthetic (plant) biology?

We would like to construct vectors to stably transform rice plants but there are so many different methods to choose from that we're not sure which method to pick (and stick to). For the moment...

09 September 2016 8,812 2 View