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Questions related from Jonas Albarnaz
Hello, I would like to know what is the best fixation (PFA? Cold methanol?) and permeabilizing agent (Triton X-100? Acetone?) for amoeba trophozoite immunostaining to detect a cytoplasmic...
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I would like to know if GM130-positive vesicles can be observed in the cytoplasm far from the Golgi complex.
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Hey everyone, I've had some problems with my protocol of nuclear fractionation and this forum was recommended. The protocol is based on hypotonic cell lysis with RBS followed by Nonidet P-40...
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Hey everyone, I've had some troubles with my protocol of nuclear fractionation and this forum was recommended. The protocol is based on hypotonic cell lysis with RBS followed by Nonidet P-40...
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