5 Questions 23 Answers 0 Followers
Questions related from Jonas Albarnaz
Hello, I would like to know what is the best fixation (PFA? Cold methanol?) and permeabilizing agent (Triton X-100? Acetone?) for amoeba trophozoite immunostaining to detect a cytoplasmic...
05 May 2016 9,279 1 View
I would like to know if GM130-positive vesicles can be observed in the cytoplasm far from the Golgi complex.
04 April 2016 3,787 1 View
I want to fuse a fluorescent protein to a viral protein in order to track its subcellular location and to assess colocalization with other proteins during infection. Since a red fluorescent...
10 October 2013 6,211 18 View
Hey everyone, I've had some problems with my protocol of nuclear fractionation and this forum was recommended. The protocol is based on hypotonic cell lysis with RBS followed by Nonidet P-40...
04 April 2011 1,342 1 View
Hey everyone, I've had some troubles with my protocol of nuclear fractionation and this forum was recommended. The protocol is based on hypotonic cell lysis with RBS followed by Nonidet P-40...
04 April 2011 2,752 7 View