Jonas De Saeger

4 Questions 8 Answers 0 Followers

Questions related from Jonas De Saeger

Which (MoClo) acceptor vector is recommended for the transfection to protoplasts?

Dear all, I would like to transfect protoplasts with constructs made using the MoClo technique, but it seems that the vectors in my toolkit are specifically made for Agrobacterium-mediated...

12 December 2016 2,563 5 View

What is best way to isolate the promoter and 5'UTR of rice genes?

I'm trying to identify the promoter and 5'UTR of a rice gene namely LOC_Os11g43830. However, I have never done this kind of identification before, I have found some results but I'm not sure if I...

09 December 2016 8,932 4 View

Does anyone have experience with isolation of rice protoplasts?

Dear all, I've been trying to isolate protoplasts from rice for quite a while now, but until now it has been unsuccessful. For a start my yield is quite low (10-fold lower than what is published...

23 October 2016 2,514 6 View

Does anyone have experience with cloning methods in synthetic (plant) biology?

We would like to construct vectors to stably transform rice plants but there are so many different methods to choose from that we're not sure which method to pick (and stick to). For the moment...

27 September 2016 4,834 2 View