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Questions related from Johnny Tam
I have two protein sequences with around 50% identity between them. I want to study the phylogenetic relationship between them. I came up with a method myself: Step 1: blast each of the sequences...
12 November 2018 3,628 2 View
I want to pull the transcription factors which binds to the biotinylated DNA probe of promoter sequence flanked 5' of a gene of interest. Any technical advice on the following questions? Probe...
18 February 2018 3,727 5 View
As title, and the following is the background FYI : I am discovering a possible single copy insertional mutation in a nonaploid plant genome. Previously I used SPades to assemble contigs of the...
23 October 2017 3,650 0 View
I have a 150bp short read whole-genome library by analyzing which I am trying to discover the breakpoint of a possible insertional mutation in a plant genome. I am wondering Is there any...
17 October 2017 7,481 2 View
I am trying to identify the point of insertion in the genome that may have caused the size difference in fruits between wild-type and mutant of a plant species (also mentioned here :...
14 October 2017 2,185 1 View
I am trying to get isolated bands from restriction digestion of a large plant genome (>800Mbp). After discussing with the people in my lab, their experience seems to have got a smear instead of...
12 September 2017 7,314 4 View