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Questions related from John Campbell McNamara
Recently, Christopher Monit, Richard A. Goldstein & Greg J. Towers published an article entitled 'ChromaClade: combined visualisation of phylogenetic and sequence data' in BMC Evolutionary...
11 November 2019 6,076 2 View
We need to preserve crab hemolymph to evaluate acid-base characteristics at a later date. Will freezing and holding in liquid nitrogen allow this? Thanks.
12 December 2015 7,857 1 View
Could anyone recommend a top journal for a manuscript on ammonia stress in vivo in freshwater shrimp gills using Na/K-, V(H+)-ATPase gene expression and activities, and oxidative enzyme activities...
05 May 2015 7,987 1 View
We are designing specific primers for qPCR analyses in a series of crustaceans. Could anyone comment on the maximum acceptable self complementarity scores please? Also on the maximum 3'...
11 November 2014 3,154 15 View
In preparation for field studies, we have compared total RNA concentrations (by Qubit) in tissues directly extracted in Trizol with tissues extracted after holding for 48 hours in RNAlater buffer....
03 March 2014 10,064 20 View
Can anyone provide a few tips on how best to separate the protein layer when extracting total RNA and DNA from tissue homogenates? We want to try western blotting using protein from the same...
03 March 2014 9,734 3 View
We will shortly be engaged in fieldwork and we need to transport tissue samples (gill, muscle) back to the lab for RNA extraction and cDNA synthesis. I know RNALater is available for this but we...
12 December 2013 2,582 11 View
Has anyone designed primers, degenerate or otherwise, for crustacean or arthropod aquaporins? We have tried a couple of our own for a Macrobrachium rosenbergii sequence (GenBank) without success....
12 December 2013 9,093 1 View
