John Palmer

2 Questions 3 Answers 0 Followers

Questions related from John Palmer

Why does my protein destain so quickly?

Hello all, The protein I'm purifying seems to destain very quickly on Coomassie stained SDS-PAGE gels (red box in image). I've heard that proline-rich proteins (like this protein) have a...

07 March 2019 269 6 View

What are the two layers after lysing bacteria?

I've lysed some BL21 cells using sonication then pelleted the insoluble fraction. On a few occasions I've seen two layers in the pellet - as shown in the photo. Does anyone have any idea what this...

14 January 2019 1,865 0 View