Why do I need Tween20 in a BrdU assay? My cells become clumpy (or lysed) and I cannot FACS them anymore.

Most Protocols for BrdU assay to determine cell cycle phases require PBS-Tween for washing and AB-incubation. I did the assay now for the first time and my cells (BxPC-3) got clumpy, or even...

07 July 2012 5,690 3 View