Johannes Schiffels

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Questions related from Johannes Schiffels

Is there an E. coli BL21(DE3)-derivative strain available, which contains chromosomal integrated extra copies of the rare tRNA-genes?

E. coli Rosetta (DE3) as well as E. coli BL21 (DE3) Codon Plus strains contain P15A-ori/CamR low copy plasmids, which provide extra copies of rare tRNA-genes. They are therefore used for...

26 August 2014 8,576 3 View

Is there an easy (routinely applicable) way to separate intact bacteria from very fine plant fibres?

Imagine an air (aerosolic) sample taken from a chicken house facility using a filter device. The aim is to collect (pathogenic) bacteria, supend them in an aqueous based solvent and use them for a...

12 May 2014 2,057 2 View