Johannes W Kung

2 Questions 4 Answers 0 Followers

Questions related from Johannes W Kung

What could I use as a loading control for a Western Blot Protease assay?

I want to quantify the activity of an unknown specimen-derived protease mix via western blot with a single standard protein. ELISA does not work because partially digested fragments will still...

25 July 2018 3,417 6 View

Sounds easy: Enzyme to het. express in E.coli (no background)

I am looking for an example of a certain protein: It should meet the following criteria: - it is an enzyme - it can be expressed heterologously in Escherichia coli in the cytoplasm (ideally shown...

01 January 1970 2,928 4 View