Johanna Sigl-Glöckner

4 Questions 3 Answers 0 Followers

Questions related from Johanna Sigl-Glöckner

Why is my PGP9.5 antibody staining much worse following paraffing embedding?

We are staining 16um sections of mouse penis with a PGP9.5 (pan-neuronal) AB. Without paraffin the staining was very nice but sections dissociated. Now we embed in paraffin but the staining is...

07 July 2018 3,548 2 View

Has anyone used a Vglu2 anti mouse antibody on mouse brain?

Is there a problem because the antibody is raised in mouse?

10 October 2016 2,940 3 View

How do I gate nuclei from debris using flow cytometry?

How do I gate a population of nuclei from debris in a FSC vs. SSC plot, or any other plot for that matter? I am analyzing a live single nuclei suspension from rat brain tissue which are stained...

09 September 2015 418 2 View

How can I paint whole chromosomes in intact rat brain slices using FISH ?

I have a whole chromosome paint for rat X chromosome but so far the staining was unsuccessful using the protocol the supplier provided for inter- and metaphase slides. Hence I am wondering how the...

03 March 2015 1,289 4 View