I'm using H2DCFDA probe to measure intracellular ROS. However I'm not able to distinguish control samples from positive control (50µM H2O2). Why?

I'm using the H2DCFDA probe to measure the intracellular ROS after a certain treatment. However, every time I measured the fluorescence intensity (with flow cytometer), my untreated samples are...

14 March 2017 4,928 5 View