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Hello, I have followed the typical protocol for a 200 mM sodium orthovanadate solution for use as a phosphatase inhibitor 100x stock: 1. Dissolve Na orthovanadate in ddH2O 2. Measure pH; it...
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Hello, I would like to use Benzonase in my lysis buffer to reduce viscosity. I have been using RIPA buffer (50mM Tris, 150mM NaCl, 0.5% Na deoxycholate, 1% NP-40, 0.1% SDS, pH 8.0) to lyse my...
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