Joel Alejandro Chuquimia

5 Questions 2 Answers 0 Followers

Questions related from Joel Alejandro Chuquimia

How to create a dataset of dengue virus for construction of phylogenetic tree?

I want to build a phylogenetic tree of data of sequencing of dengue, but I don't have experience in that, I had had the experience in SARS-CoV-2, in that case I used my sequences (query), the...

24 August 2023 7,619 1 View

How to synthesize a primer and probe for qPCR real time in vitro?

Hello everyone I would like to know if someone have experience in the process of synthesis of primers and probes for a qPCR real time (in vitro), for this process, what type of material or...

16 June 2022 7,788 0 View

What cycling stage should I put for melt curve?

I want to evaluate the specificity and development of secondaries structures of my primers and probes against dengue virus (I designed them), I don't know what the usually temperatures for this...

03 June 2022 1,622 3 View

What are the parameters to validate a pair of primers and probes in a singleplex real time RT-PCR?

Hello, I would like to know your opinion about it: apart from checking the amplification curve, what parameters are considered to validate the primers in a singleplex type real-time RT-PCR? I...

25 December 2021 980 2 View

How to create a Melt curve in qTOWER3 (qPCRsoft touch)?

Hello everyone recently I got a qTOWER3 (a termocycler for qPCR) so, I do not have a lot of experience in this machine and I would ask you about set up a Melt curve. Firstly, I have a assay for...

23 November 2021 1,189 0 View