Why are my Balb/C 3T3 cells detaching from the 96 well microplate during incubation in EBSS in the 3T3 NRU phototoxicity test?

Hello, I am currently working on the 3T3 NRU phototoxicity test (OECD 432). However, during the 1hr 50 min incubation in EBSS buffer the cells are detaching from the microplate. Has anyone else...

17 November 2021 4,557 1 View