Job Alves de Souza Fh

4 Questions 4 Answers 0 Followers

Questions related from Job Alves de Souza Fh

What is the best method for concentration and purification of viral particles (Sars-Cov-2 in this case)?

Ion-exchange Chromatography (IEC) and Size Exclusion Chromatography (SEC) method or PEG precipitation followed by sucrose density gradient? For use as an antigen, for example, it would be...

22 May 2020 7,066 4 View

Could the discovery of Trypomastigote of Trypanosoma cruzi in bone marrow be genuine or due to contamination?

Dear colleaguesI found a trypomastigote of Trypanosoma cruzi in the bone marrow aspirate from a patient. Although the sample is very characteristic of marrow, I'm afraid that may have occurred...

14 April 2015 9,716 4 View

Can anyone tell me what is the best medium for isolation of Leishmania without addition of blood?

Between RPMI and Schneider what is the best? My purpose would be to isolate Leishmania infantum from dogs.

26 January 2015 7,718 5 View

Why does BFS inhibit IL-1β detection?

In a culture of Bone Marrow Derived Macrophage (BMDM) the supplementation of the medium with 10% of Bovine Fetal Serum (BFS) inhibits the detection of IL-1β by ELISA, but not of other cytokines....

10 January 2013 9,220 4 View