João Coelho

5 Questions 13 Answers 0 Followers

Questions related from João Coelho

Should a MtMam model of protein evolution be chosen for a non-mitochondrial protein?

I ran ProtTest3.0 using my alignment of viral protein sequences. I tested all available models, and the best ones were MtMam based (they differ in the parameters), an HIV one and only then does...

11 November 2013 1,686 1 View

Program to quantify differences between aligned sequences?

Does anyone know a program that quantifies and displays (like in a table format) the differences between aligned sequences? For ex. I've got 14 aligned DNA sequences, and I'd like to know how many...

10 October 2012 1,404 11 View

Has anyone expressing proteins in S. cerevisiae ever encountered insoluble proteins in that system?

I'm expressing a protein in S. cerevisiae, and I see it when I do a crude total extract. However, when I refine the protein extraction method, in order to obtain soluble proteins, I do not find my...

07 July 2012 8,458 4 View

Agarose concentration for separation of large DNA fragments of similar size?

in a 1% agarose gel, at 20V, the band are still very close and I'm still unable to cut one of them from

06 June 2012 3,292 7 View

Agarose concentration for separation of large DNA fragments of similar size?

I'm trying to separate a 3700bp fragment from a 3600bp one. However, after running them for 24h in a 1% agarose gel, at 20V, the band are still very close and I'm still unable to cut one of them...

06 June 2012 8,503 33 View