Joanna Wardyn

2 Questions 7 Answers 0 Followers

Questions related from Joanna Wardyn

Should I supplement Neurobasal medium with serum for comparative experiments on hippocampal astrocytes?

I have done a lot of live cell imaging on hippocampal neuron-astrocyte cultures in Neurobasal medium and now I would like to look at astrocytes in pure culture. I normally culture the astrocytes...

24 March 2015 7,904 6 View

How can one estimate lentiviral MOIs using fluorescent microscope?

I have made multiple lentiviruses expressing fluorescent protein fusions, which I need to titrate. Unfortunately counting infected cells with FACS is not possible as some of my viruses are...

11 February 2015 8,004 3 View