Does anybody know why an in vitro synthesized RNA transcript (~1000 bp) might migrate through non-denaturating 6 % PAGE gel in the form of dumbbell bands?

These bands have what seem like aggregates of RNA in the middle, which cause them to smear/smudge during migration. I found an example of that phenomenon online, which pretty much reflects what I...

15 February 2013 8,635 11 View