Joanna Ewa Sowa

2 Questions 2 Answers 0 Followers

Questions related from Joanna Ewa Sowa

What is the best way to measure NMDA currents in basolateral amygdala?

Hello, I've been trying to measure NMDA currents in the BLA. I've been using magnesium free acsf (122 NaCl, 2 KCl, 1.25 KH2PO4, 26 NaHCO3, 2.5 CaCl2, 10 D-glucose with nbqx (5uM) and bicuculine...

31 October 2017 9,213 1 View

How to perform good whole-cell patch-clamp recording on primary cultures?

I'm trying to perform whole-cell patch-clamp on primary astroglia cultures and I've noticed a very narrow time window to get good seels (aprox. 3-4 days after isolation). One day I have nice cells...

10 February 2017 230 3 View