How do I get DAPI to work after 4N HCl treatment of paraffin sections?

Hi. I am doing an immunofluorescence on paraffin sections using 5hmC and 5mC antibodies. I have managed to get them to work after antigen retrieval and incubation in 4N HCl, however my DAPI didn't...

17 November 2016 5,192 3 View