Joanna Marian Gutiérrez

2 Questions 3 Answers 0 Followers

Questions related from Joanna Marian Gutiérrez

Why am I having false positives in my transformants in E.Coli?

Hi, I'm having trouble with my tranformation. I'm currently trying to do a subclonning with plasmid PBC sk(+), the thing is that I'm having colonies and they are positive in colony PCR but when I...

01 April 2025 9,194 7 View

Why I'm losing plasmid after digest with restriction enzymes?

Hi everyone, I'm currently working with a plasmid called pBI101, I isolated this plasmid from a plate then I did the extraction with mini prep protocol and cut it with SmaI at 25°C incubation...

18 November 2023 7,028 3 View