Why can I observe no insulin secretion peak 2 min after intraperitoneal injection of 3 g/kg in mice?

This is a problem we are having lately in my lab. We collect blood samples with EDTA Microvettes, centrifuge 3500rpm 15 min 4ºC, obtain the plasma, and analyse insulin concentration with ELISA...

24 February 2015 5,088 10 View