Joan Chang

3 Questions 11 Answers 0 Followers

Questions related from Joan Chang

PCR product doesn't appear on gel but nanodrop suggested high concentration?

I did a standard KAPA HiFi Hotstart PCR reaction, including a positive control that I know has worked before in the cycling conditions I've used (basically what was recommended by the...

07 January 2017 6,557 7 View

I've come across the inhibitor 'GW4869' where it was described as exosome inhibitor - how specific is this?

In the sense that does it inhibit release of other types of extracellular vesicle? Thanks!

28 May 2015 5,624 7 View

Can anyone help with an odd running pattern on SDS-PAGE?

Disclaimer: I have run many SDS-PAGE/Western blots before and this has happened to me twice in the last couple of days. I lysed less than 60000 cells in 70ul RIPA buffer, sonicated the samples...

23 April 2014 9,218 10 View