Can too much of Tris-Cl buffer inhibit PCR?

I accidentally resuspended my stock primers (100 uM) in tris-Cl (pH 8.0) 1 M instead of 10 mM. I do PCR with 20 uM dilution from the stock. Will the high concentration of Tris-Cl inhibit PCR? I...

13 July 2020 3,189 10 View