Jingyi Liu

2 Questions 6 Answers 0 Followers

Questions related from Jingyi Liu

I can't detect signals using pAkt thr 308 for whole mount ICC. What should I do to solve this problem?

I performed whole mount ICC on zebra fish embryos using a pAkt antibody. I couldn't get signals, at least no good signals. The background is pretty high. I searched online protocols. I thought it...

10 October 2014 433 6 View

How could I get a sharp band when doing WB?

I've done Western Blotting using total Akt, pAkt ser 473 and pAkt thr 308. For total Akt and pAkt ser 473, I mostly see a very clear and sharp band. But there are so many non-specific bands for...

10 October 2014 4,095 4 View