Jing Zhu

2 Questions 4 Answers 0 Followers

Questions related from Jing Zhu

PCR. For double-stranded DNA template. Instead of the the two strands bind each other, the lower Tm primers bind one strand?

Why primer binding template instead of the two strands bind each other? The Tm of one strand is higher than primers. Complex structure of the template? The amount of primer is much higher than...

29 November 2022 5,741 1 View

Noise signal are very high when multiplex RT-PCR using 4 taqman probes FAM/ROX/HEX/CY5 ?

All components are freeze-dried. We use AB7500. compare the liquid state, the other channels' noise signal of freeze-dried components are very high when we only detect one channel

22 December 2015 6,011 1 View