Jing-Mei Qian

3 Questions 7 Answers 0 Followers

Questions related from Jing-Mei Qian

Can I use error-prone PCR to do random mutagenesis in 5 kb fragments ?

I want to do random mutagenesis in 5 kb fragments. I want to use the product to do directed evolution. I used two error-prone PCR kits (Aligent and Takara) and can't amplify fragments which size...

30 October 2022 3,210 4 View

Should I use constitutive promoters or inducible promoters when express three genes in E.coli?

Hi, I want to express three genes in E. coli. I have seen some papers which used inducible promoters, like IPTG promoter or Arabinose promoter, to express their genes heterologously. But, I have...

14 May 2022 7,643 3 View

Why don't use lysogenic phage as a tool to transfer plasmid in lab?

Recently, I'm working on a bacteria isolated from soil. It's hard to transfom. I have used chemical transformation and electrotransformation methods. They all failed. Then I found phage...

29 April 2022 7,179 4 View