Jing-Mei Qian

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Questions related from Jing-Mei Qian

Can I use error-prone PCR to do random mutagenesis in 5 kb fragments ?

I want to do random mutagenesis in 5 kb fragments. I want to use the product to do directed evolution. I used two error-prone PCR kits (Aligent and Takara) and can't amplify fragments which size...

30 October 2022 3,154 4 View

Should I use constitutive promoters or inducible promoters when express three genes in E.coli?

Hi, I want to express three genes in E. coli. I have seen some papers which used inducible promoters, like IPTG promoter or Arabinose promoter, to express their genes heterologously. But, I have...

14 May 2022 7,609 3 View