Jindou Dong

4 Questions 3 Answers 0 Followers

Questions related from Jindou Dong

How to concentrate proteins that tend to adhere to the membrane of centrifugal filter concentrators ?

The concentration of recombinant proteins was generally unsuccessful as they tend to adhere to the membrane except in high salt buffer(>500mM NaCl) which would have interfered with the...

12 May 2016 8,121 6 View

How does one calculate the protein complex concentration?

How to use BCA assay calculate the concentration of the protein complex by using only 3 ul of the sample ? thanks guys , the protocol I followed doesn't work at all .

12 July 2014 4,092 2 View

How would the addition of a metal effect the over expression of a protein?

The E3 RNF8 is a zinc binding protein. Will the addition of the zinc ion increase the solubility of the RNF8 protein?

02 July 2014 8,805 1 View

What is the optimal induction time at 16 degrees?

I want to optimize the protein expression by inducing at a low temperature. I am just wondering what is the best induction time at 16 degree. I am using BL21 and 0.5 M IPTG. Thank you guys.

30 June 2014 3,401 5 View