Jin Yang

2 Questions 4 Answers 0 Followers

Questions related from Jin Yang

Why the T cell transducing rate is way lower with lower titer-ed virus comparing to the higher titer-ed virus??

I produce 2nd generation lentivirus with 293T cells for T cell transduction. We concentrated our virus through 10000g centrifuge overnight. After I obtained my concentrated virus, I titrate my...

09 November 2020 3,430 4 View

What's my problem?

I need to concentrat my lentivirus (2nd generation) to transduce the T cells. I can have reasonable amount of total virions estimated from the crude titration, but the concentrated virus titer is...

20 December 2019 3,963 3 View